NXF3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NXF3 transfected lysate.
Western Blot
Lane 1: NXF3 transfected lysate ( 60.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NXF3 full-length
Host
Human
Theoretical MW (kDa)
60.1
Interspecies Antigen Sequence
Mouse (51); Rat (51)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NXF3 antibody (H00056000-B01) by Western Blots.
SDS-PAGE Gel
NXF3 transfected lysate.
Western Blot
Lane 1: NXF3 transfected lysate ( 60.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NXF3
Entrez GeneID
56000GeneBank Accession#
NM_022052Protein Accession#
NP_071335Gene Name
NXF3
Gene Alias
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Gene Description
nuclear RNA export factor 3
Omim ID
300316Gene Ontology
HyperlinkGene Summary
This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus. [provided by RefSeq
Other Designations
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