NSFL1C 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
NSFL1C transfected lysate.
Western Blot
Lane 1: NSFL1C transfected lysate ( 40.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NSFL1C full-length
Host
Human
Theoretical MW (kDa)
40.6
Interspecies Antigen Sequence
Mouse (95); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NSFL1C antibody (H00055968-B01) by Western Blots.
SDS-PAGE Gel
NSFL1C transfected lysate.
Western Blot
Lane 1: NSFL1C transfected lysate ( 40.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NSFL1C
Entrez GeneID
55968GeneBank Accession#
NM_016143Protein Accession#
NP_057227Gene Name
NSFL1C
Gene Alias
MGC3347, UBX1, UBXD10, UBXN2C, dJ776F14.1, p47
Gene Description
NSFL1 (p97) cofactor (p47)
Omim ID
606610Gene Ontology
HyperlinkGene Summary
N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000029972|OTTHUMP00000029974|SHP1 homolog|UBX domain protein 2C|p47 protein
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