ZNF313 monoclonal antibody (M01), clone 4G3-1A10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ZNF313.
Immunogen
ZNF313 (AAH13695, 1 a.a. ~ 228 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAQQRDCGGAAQLAGPAAEADPLGRFTCPVCLEVYEKPVQVPCGHVFCSACLQECLKPKKPVCGVCRSALAPGVRAVELERQIESTETSCHGCRKNFFLSKIRSHVATCSKYQNYIMEGVKATIKDASLQPRNVPNRYTFPCPYCPEKNFDQEGLVEHCKLFHSTDTKSVVCPICASMPWGDPNYRSANFREHIQRRHRFSYDTFVDYDVDEEDMMNQVLQRSIIDQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (89); Rat (88)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (50.82 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ZNF313 monoclonal antibody (M01), clone 4G3-1A10 Western Blot analysis of ZNF313 expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of ZNF313 expression in transfected 293T cell line by ZNF313 monoclonal antibody (M01), clone 4G3-1A10.
Lane 1: ZNF313 transfected lysate(25.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ZNF313 on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ZNF313 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — RNF114
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Interactome
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Disease
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Publication Reference
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Functional analysis of the RNF114 psoriasis susceptibility gene implicates innate immune responses to double-stranded RNA in disease pathogenesis.
Bijlmakers MJ, Kanneganti SK, Barker JN, Trembath RC, Capon F.
Human Molecular Genetics 2011 Aug; 20(16):3129.
Application:IF, WB-Ce, WB-Tr, Human, HaCaT, HEK293T cells.
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Functional analysis of the RNF114 psoriasis susceptibility gene implicates innate immune responses to double-stranded RNA in disease pathogenesis.
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