HDAC8 monoclonal antibody (M01), clone 2F4
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More Files
- More Functions
Western Blot (Cell lysate)
HDAC8 monoclonal antibody (M01), clone 2F4 Western Blot analysis of HDAC8 expression in Hela S3 NE ( Cat # L013V3 ).
Western Blot (Transfected lysate)
Western Blot analysis of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M01), clone 2F4.
Lane 1: HDAC8 transfected lysate(41.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HDAC8 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to HDAC8 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (66.99 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant HDAC8.
Immunogen
HDAC8 (AAH50433, 1 a.a. ~ 377 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEEPEEPADSGQSLVPVYIYSPEYVSMCDSPAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGYDCPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPHRIQQILNYIKGNLKHVV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (66.99 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HDAC8 monoclonal antibody (M01), clone 2F4 Western Blot analysis of HDAC8 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M01), clone 2F4.
Lane 1: HDAC8 transfected lysate(41.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HDAC8 is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to HDAC8 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — HDAC8
Entrez GeneID
55869GeneBank Accession#
BC050433Protein Accession#
AAH50433Gene Name
HDAC8
Gene Alias
HDACL1, RPD3
Gene Description
histone deacetylase 8
Omim ID
300269Gene Ontology
HyperlinkGene Summary
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. [provided by RefSeq
Other Designations
histone deacetylase-like 1
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Interactome
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Disease
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Publication Reference
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The transcription regulation mechanism of the syntaxin 1A gene via protein kinase A.
Nakayama T, Akagawa K.
The Biochemical Journal 2017 May; 474(14):2465.
Application：WB-Ce, Rat, FRSK cells.
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The transcription regulation mechanism of the syntaxin 1A gene via protein kinase A.
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