CENPJ 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CENPJ transfected lysate.
Western Blot
Lane 1: CENPJ transfected lysate ( 43.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CENPJ full-length
Host
Human
Theoretical MW (kDa)
43.2
Interspecies Antigen Sequence
Mouse (83); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CENPJ antibody (H00055835-B01) by Western Blots.
SDS-PAGE Gel
CENPJ transfected lysate.
Western Blot
Lane 1: CENPJ transfected lysate ( 43.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CENPJ
Entrez GeneID
55835GeneBank Accession#
BC024209.2Protein Accession#
-Gene Name
CENPJ
Gene Alias
BM032, CPAP, LAP, LIP1, MCPH6, MGC131581, MGC131582, MGC142222, MGC142224
Gene Description
centromere protein J
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation. [provided by RefSeq
Other Designations
LAG-3-associated protein|LYST-interacting protein LIP1|LYST-interacting protein LIP7|OTTHUMP00000018137|centrosomal P4.1-associated protein
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Interactome
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Disease
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