CENPJ monoclonal antibody (M01), clone 5D5
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CENPJ on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 1.5 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CENPJ is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (37.84 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CENPJ.
Immunogen
CENPJ (NP_060921, 979 a.a. ~ 1088 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KYTTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (83); Rat (81)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CENPJ on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 1.5 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CENPJ is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — CENPJ
Entrez GeneID
55835GeneBank Accession#
NM_018451Protein Accession#
NP_060921Gene Name
CENPJ
Gene Alias
BM032, CPAP, LAP, LIP1, MCPH6, MGC131581, MGC131582, MGC142222, MGC142224
Gene Description
centromere protein J
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation. [provided by RefSeq
Other Designations
LAG-3-associated protein|LYST-interacting protein LIP1|LYST-interacting protein LIP7|OTTHUMP00000018137|centrosomal P4.1-associated protein
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Interactome
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Disease
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