MBD5 monoclonal antibody (M01), clone 4A12-1B6
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant MBD5.
Immunogen
MBD5 (AAH14534.1, 1 a.a. ~ 229 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALRKRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQGQGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASNTALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCHNANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLSQSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSYNQTSSEAGMVLLEKSTQRY
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (50.93 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MBD5 expression in transfected 293T cell line by MBD5 monoclonal antibody (M01), clone 4A12-1B6.
Lane 1: MBD5 transfected lysate(24.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MBD5 is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MBD5 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MBD5
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Interactome
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