RC74 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
INTS9 transfected lysate.
Western Blot
Lane 1: INTS9 transfected lysate ( 73.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RC74 full-length
Host
Human
Theoretical MW (kDa)
73.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RC74 antibody (H00055756-B01) by Western Blots.
SDS-PAGE Gel
INTS9 transfected lysate.
Western Blot
Lane 1: INTS9 transfected lysate ( 73.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — INTS9
Entrez GeneID
55756GeneBank Accession#
BC025267Protein Accession#
AAH25267.1Gene Name
INTS9
Gene Alias
CPSF2L, FLJ10871, INT9, RC-74, RC74
Gene Description
integrator complex subunit 9
Omim ID
611352Gene Ontology
HyperlinkGene Summary
INTS9 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM
Other Designations
related to CPSF subunits 74 kDa
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