CHFR monoclonal antibody (M01), clone 1H3-A12
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More Files
- More Functions
Western Blot (Cell lysate)
CHFR monoclonal antibody (M01), clone 1H3-A12. Western Blot analysis of CHFR expression in 293.
Western Blot (Transfected lysate)
Western Blot analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.
Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (97.46 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant CHFR.
Immunogen
CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (80); Rat (80)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (97.46 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
CHFR monoclonal antibody (M01), clone 1H3-A12. Western Blot analysis of CHFR expression in 293.Western Blot (Transfected lysate)
Western Blot analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.
Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — CHFR
Entrez GeneID
55743GeneBank Accession#
BC012072Protein Accession#
AAH12072.1Gene Name
CHFR
Gene Alias
FLJ10796, FLJ33629, RNF116, RNF196
Gene Description
checkpoint with forkhead and ring finger domains
Omim ID
605209Gene Ontology
HyperlinkOther Designations
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Interactome
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Disease
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Publication Reference
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Stabilization of HDAC1 via TCL1-pAKT-CHFR axis is a key element for NANOG-mediated multi-resistance and stem-like phenotype in immune-edited tumor cells.
Woo SR, Lee HJ, Oh SJ, Kim S, Park SH, Lee J, Song KH, Kim TW.
Biochemical and Biophysical Research Communications 2018 Sep; 503(3):1812.
Application：WB-Tr, Human, CaSki, CaSki-NANOG, HEK293, HeLa cells.
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Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers.
Zhang D, Xu XL, Li F, Sun HC, Cui YQ, Liu S, Xu PY.
Oncology Letters 2017 Dec; 14(6):8042.
Application：IHC-P, Human, Human pancreatic cancer.
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CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation.
Kashima L, Idogawa M, Mita H, Shitashige M, Yamada T, Ogi K, Suzuki H, Toyota M, Ariga H, Sasaki Y, Tokino T.
The Journal of Biological Chemistry 2012 Apr; 287(16):12975.
Application：IP, WB-Tr, Human, HCT-116, HSC-44, MKN45 cells.
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Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach.
Fujita A, Yamamoto H, Imamura M, Nakamura N, Maehara Y, Tsuneyoshi M, Oda Y.
Virchows Archiv 2011 Dec; 460(2):163.
Application：IHC, Human, Human gastrointestinal stromal tumors.
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Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer.
Hu SL, Huang DB, Sun YB, Wu L, Xu WP, Yin S, Chen J, Jiang XD, Shen G.
Medical Oncology 2011 Jun; 28(2):447.
Application：IHC-P, Human, Gastric cancer.
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Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma.
Gao YJ, Xin Y, Zhang JJ, Zhou J.
World Journal of Gastroenterology 2008 Aug; 14(32):5000.
Application：IHC-P, Human, Gastric carcinoma tissue samples, Normal gastric mucosa samples.
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Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint.
Privette LM, Weier JF, Nguyen HN, Yu X, Petty EM.
Neoplasia 2008 Jul; 10(7):643.
Application：IF, WB, Human, MCF10A cells.
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Altered Expression of the Early Mitotic Checkpoint Protein, CHFR, in Breast Cancers: Implications for Tumor Suppression.
Privette LM, Gonzalez ME, Ding L, Kleer CG, Petty EM.
Cancer Research 2007 Jun; 67(13):6064.
Application：IHC-P, WB, Human, Human breast cancer, MCF10A cells.
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Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis.
Milne AN, Sitarz R, Carvalho R, Polak MM, Ligtenberg M, Pauwels P, Offerhaus GJ, Weterman MA.
Human pathology 2007 Mar; 38(6):903.
Application：IHC-P, Human, Human gastric cancer.
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Stabilization of HDAC1 via TCL1-pAKT-CHFR axis is a key element for NANOG-mediated multi-resistance and stem-like phenotype in immune-edited tumor cells.
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