EDEM2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
EDEM2 transfected lysate.
Western Blot
Lane 1: EDEM2 transfected lysate ( 64.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EDEM2 full-length
Host
Human
Theoretical MW (kDa)
64.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EDEM2 antibody (H00055741-D01P) by Western Blots.
SDS-PAGE Gel
EDEM2 transfected lysate.
Western Blot
Lane 1: EDEM2 transfected lysate ( 64.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EDEM2
Entrez GeneID
55741GeneBank Accession#
BC001371.2Protein Accession#
AAH01371.1Gene Name
EDEM2
Gene Alias
C20orf31, C20orf49, FLJ10783, bA4204.1
Gene Description
ER degradation enhancer, mannosidase alpha-like 2
Omim ID
610302Gene Ontology
HyperlinkGene Summary
In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).[supplied by OMIM
Other Designations
ER degradation-enhancing-mannosidase-like protein 2|OTTHUMP00000030728
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