ARFGAP1 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human ARFGAP1 full-length ORF ( AAH28233, 1 a.a. - 414 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKFWGHKQQPEPASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
71.28
Interspecies Antigen Sequence
Mouse (83); Rat (82)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — ARFGAP1
Entrez GeneID
55738GeneBank Accession#
BC028233Protein Accession#
AAH28233Gene Name
ARFGAP1
Gene Alias
ARF1GAP, HRIHFB2281, MGC39924
Gene Description
ADP-ribosylation factor GTPase activating protein 1
Omim ID
608377Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ADP-ribosylation factor 1 GTPase activating protein|ARF1-directed GTPase-activating protein|GAP protein|OTTHUMP00000031551
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Interactome
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Pathway
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