ARFGAP1 mouse monoclonal antibody (hybridoma)
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant ARFGAP1.
Immunogen
ARFGAP1 (NP_060679.1, 1 a.a. ~ 406 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MASPRTRKVLKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESSPAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLNDDLGSYQGAQGNRYVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSASTNRNSNSDGGEGGEGTKKAVPPAVPTDDGWDNQNW
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (86); Rat (84)
Quality Control Testing
Antibody reactivity and specificity confirmed by ELISA and Western Blot.
Deliverables
Up to 5 positive hybridoma clones will be delivered to customer in the cryotube format.
Note
Customer should check the viability of the hybridomas within one month from the date of receipt. Fee-for-service of long term hybridoma storage can be performed upon customer's request.
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Applications
Western Blot (Transfected lysate)
Western Blot (Recombinant protein)
ELISA
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Gene Info — ARFGAP1
Entrez GeneID
55738GeneBank Accession#
NM_018209.2Protein Accession#
NP_060679.1Gene Name
ARFGAP1
Gene Alias
ARF1GAP, HRIHFB2281, MGC39924
Gene Description
ADP-ribosylation factor GTPase activating protein 1
Omim ID
608377Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ADP-ribosylation factor 1 GTPase activating protein|ARF1-directed GTPase-activating protein|GAP protein|OTTHUMP00000031551
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Interactome
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Pathway
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