VAC14 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
VAC14 transfected lysate.
Western Blot
Lane 1: VAC14 transfected lysate ( 88 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-VAC14 full-length
Host
Human
Theoretical MW (kDa)
88
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-VAC14 antibody (H00055697-B01) by Western Blots.
SDS-PAGE Gel
VAC14 transfected lysate.
Western Blot
Lane 1: VAC14 transfected lysate ( 88 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — VAC14
Entrez GeneID
55697GeneBank Accession#
NM_018052.3Protein Accession#
-Gene Name
VAC14
Gene Alias
ArPIKfyve, FLJ10305, FLJ36622, FLJ46582, MGC149815, MGC149816, TAX1BP2, TRX
Gene Description
Vac14 homolog (S. cerevisiae)
Omim ID
604632Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling molecule. A regulatory complex made up of VAC14 and FIG4 (MIM 609390) control synthesis of PI(3,5)P2 by activating PI(3)P kinase, FAB1 (PIP5K3; MIM 609414). The VAC14/FIG4 complex also functions in the breakdown of PI(3,5)P2 (Zhang et al., 2007 [PubMed 17956977]).[supplied by OMIM
Other Designations
Tax1 (human T-cell leukemia virus type I) binding protein 1|Tax1 (human T-cell leukemia virus type I) binding protein 2|Vac14 homolog
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Interactome
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Disease
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