VAC14 monoclonal antibody (M03), clone 3B2
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant VAC14.
Immunogen
VAC14 (NP_060522.3, 714 a.a. ~ 782 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SHRLQCVPNPELLQTEDSLKAAPKSQKADSPSIDYAELLQHFEKVQNKHLEVRHQRSGRGDHLDRRVVL
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (33.33 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of VAC14 expression in transfected 293T cell line by VAC14 monoclonal antibody (M03), clone 3B2.
Lane 1: VAC14 transfected lysate (Predicted MW: 88 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VAC14 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — VAC14
Entrez GeneID
55697GeneBank Accession#
NM_018052Protein Accession#
NP_060522.3Gene Name
VAC14
Gene Alias
ArPIKfyve, FLJ10305, FLJ36622, FLJ46582, MGC149815, MGC149816, TAX1BP2, TRX
Gene Description
Vac14 homolog (S. cerevisiae)
Omim ID
604632Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling molecule. A regulatory complex made up of VAC14 and FIG4 (MIM 609390) control synthesis of PI(3,5)P2 by activating PI(3)P kinase, FAB1 (PIP5K3; MIM 609414). The VAC14/FIG4 complex also functions in the breakdown of PI(3,5)P2 (Zhang et al., 2007 [PubMed 17956977]).[supplied by OMIM
Other Designations
Tax1 (human T-cell leukemia virus type I) binding protein 1|Tax1 (human T-cell leukemia virus type I) binding protein 2|Vac14 homolog
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Interactome
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Disease
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