C9orf86 monoclonal antibody (M01), clone 2E11-1F10
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant C9orf86.
Immunogen
C9orf86 (AAH02945, 1 a.a. ~ 477 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKIVIRGDRNTGKTALWHRLQGRPFVEEYIPTQEIQVTSIHWSYKTTDDIVKVEVWDVVDKGKCKKRGDGLKMENDPQEAESEMALDAEFLDVYKNCNGVVMMFDITKQWTFNYILRELPKVPTHVPVCVLGNYRDMGEHRVILPDDVRDFIDNLDRPPGSSYFRYAESSMKNSFGLKYLHKFFNIPFLQLQRETLLRQLETNQLDMDATLEELSVQQETEDQNYGIFLEMMEARSRGHASPLAANGQSPSPGSQSPVVPAGAVSTGSSSPGTPQPAPQLPLNAAPPSSVPPVPPSEALPPPACPSAPAPRRSIISRPPPWGWRLRGALGRRGQWPPWGGGRACHCLGRHLPLYHRLCRCPVAAVCASELEEAGHWWSPGWALQVLGLQAQCEPALQEGRGQLASARLGGHPGPLGAEPPVFLRDVTEAQEGPVRVCLQRLGRGRLAVGCALPRHLLALRAHLGPQHAYGSASGREP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (87); Rat (89)
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (78.21 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged C9orf86 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to C9orf86 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — C9orf86
Entrez GeneID
55684GeneBank Accession#
BC002945Protein Accession#
AAH02945Gene Name
C9orf86
Gene Alias
FLJ10101, FLJ13045, Parf, RBEL1, bA216L13.9, pp8875
Gene Description
chromosome 9 open reading frame 86
Omim ID
610615Gene Ontology
HyperlinkOther Designations
Rab-like GTP-binding protein 1|partner of ARF
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Interactome
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Publication Reference
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Down-regulation of c9orf86 in human breast cancer cells inhibits cell proliferation, invasion and tumor growth and correlates with survival of breast cancer patients.
Li YY, Fu S, Wang XP, Wang HY, Zeng MS, Shao JY.
PLoS One 2013 Aug; 8(8):e71764.
Application:IHC, WB-Ce, WB-Tr, Human, MCF-10A, MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468, SK-BR-3 cells, Breast cancer.
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Down-regulation of c9orf86 in human breast cancer cells inhibits cell proliferation, invasion and tumor growth and correlates with survival of breast cancer patients.
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