MFN1 monoclonal antibody (M04J), clone 3C9
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More Files
- More Functions
Western Blot (Cell lysate)
MFN1 monoclonal antibody (M04J), clone 3C9. Western Blot analysis of MFN1 expression in K-562.
Western Blot (Transfected lysate)
Western Blot analysis of MFN1 expression in transfected 293T cell line by MFN1 monoclonal antibody (M04J), clone 3C9.
Lane 1: MFN1 transfected lysate (Predicted MW: 84.2 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MFN1 is 3 ng/ml as a capture antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MFN1 is 0.1 ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of MFN1 over-expressed 293 cell line, cotransfected with MFN1 Validated Chimera RNAi ( Cat # H00055669-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MFN1 monoclonal antibody (M04), clone 3C9 (Cat # H00055669-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (107.25 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant MFN1.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
MFN1 (AAH40557, 1 a.a. ~ 741 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAEPVSPLKHFVLAKKAITAIFDQLLEFVTEGSHFVEATYKNPELDRIATEDDLVEMQGYKDKLSIIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVRVFWPKAKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVNALEAQNRIFFVSAKEVLSARKQKAQGMPESGVALAEGFHARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILATVKNIMDSVNLAAEDKRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQDKLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLASTPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYERLSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEEEIARLPKEIDQLEKIQNNSKLLRNKAVQLENELENFTKQFLPSSNEES
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (91); Rat (91)
Preparation Method
Cell Culture Production
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (107.25 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MFN1 monoclonal antibody (M04J), clone 3C9. Western Blot analysis of MFN1 expression in K-562.Western Blot (Transfected lysate)
Western Blot analysis of MFN1 expression in transfected 293T cell line by MFN1 monoclonal antibody (M04J), clone 3C9.
Lane 1: MFN1 transfected lysate (Predicted MW: 84.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MFN1 is 3 ng/ml as a capture antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MFN1 is 0.1 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MFN1 over-expressed 293 cell line, cotransfected with MFN1 Validated Chimera RNAi ( Cat # H00055669-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MFN1 monoclonal antibody (M04), clone 3C9 (Cat # H00055669-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MFN1
Entrez GeneID
55669GeneBank Accession#
BC040557Protein Accession#
AAH40557Gene Name
MFN1
Gene Alias
DKFZp762F247, FLJ20693, MGC41806, hfzo1, hfzo2
Gene Description
mitofusin 1
Omim ID
608506Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq
Other Designations
mitochondrial transmembrane GTPase FZO-2|mitochondrial transmembrane GTPase Fzo-1|putative transmembrane GTPase
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Interactome
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Disease
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