MED29 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MED29 transfected lysate.
Western Blot
Lane 1: MED29 transfected lysate ( 23.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MED29 full-length
Host
Human
Theoretical MW (kDa)
23.5
Interspecies Antigen Sequence
Mouse (95); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MED29 antibody (H00055588-B01) by Western Blots.
SDS-PAGE Gel
MED29 transfected lysate.
Western Blot
Lane 1: MED29 transfected lysate ( 23.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MED29
Entrez GeneID
55588GeneBank Accession#
NM_017592Protein Accession#
NP_060062Gene Name
MED29
Gene Alias
DKFZp434H247, IXL
Gene Description
mediator complex subunit 29
Gene Ontology
HyperlinkGene Summary
MED29 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 [PubMed 14576168]).[supplied by OMIM
Other Designations
intersex-like
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