CHST12 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CHST12 transfected lysate.
Western Blot
Lane 1: CHST12 transfected lysate ( 45.65 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CHST12 full-length
Host
Human
Theoretical MW (kDa)
45.65
Interspecies Antigen Sequence
Mouse (83); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CHST12 antibody (H00055501-B01) by Western Blots.
SDS-PAGE Gel
CHST12 transfected lysate.
Western Blot
Lane 1: CHST12 transfected lysate ( 45.65 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CHST12
Entrez GeneID
55501GeneBank Accession#
NM_018641.3Protein Accession#
NP_061111.1Gene Name
CHST12
Gene Alias
C4S-2, C4ST-2, C4ST2
Gene Description
carbohydrate (chondroitin 4) sulfotransferase 12
Omim ID
610129Gene Ontology
HyperlinkGene Summary
Chondroitin 4-sulfotransferases, such as CHST12, add sulfate to position 4 of N-acetylgalactosamine residues in chondroitin (Hiraoka et al., 2000 [PubMed 10781601]).[supplied by OMIM
Other Designations
OTTHUMP00000025467|chondroitin 4-O-sulfotransferase 2
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