CHST12 monoclonal antibody (M01), clone 3D6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of CHST12 expression in transfected 293T cell line by CHST12 monoclonal antibody (M01), clone 3D6.
Lane 1: CHST12 transfected lysate (Predicted MW: 48.4 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CHST12 is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.19 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CHST12.
Immunogen
CHST12 (NP_061111.1, 56 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RDRELTADSDVDEFLDKFLSAGVKQSDLPRKETEQPPAPGSMEESVRGYDWSPRDARRSPDQGRQQAERRSVLRGFCANSSLAFPTKERAFDDIP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (65); Rat (64)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.19 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CHST12 expression in transfected 293T cell line by CHST12 monoclonal antibody (M01), clone 3D6.
Lane 1: CHST12 transfected lysate (Predicted MW: 48.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CHST12 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — CHST12
Entrez GeneID
55501GeneBank Accession#
NM_018641Protein Accession#
NP_061111.1Gene Name
CHST12
Gene Alias
C4S-2, C4ST-2, C4ST2
Gene Description
carbohydrate (chondroitin 4) sulfotransferase 12
Omim ID
610129Gene Ontology
HyperlinkGene Summary
Chondroitin 4-sulfotransferases, such as CHST12, add sulfate to position 4 of N-acetylgalactosamine residues in chondroitin (Hiraoka et al., 2000 [PubMed 10781601]).[supplied by OMIM
Other Designations
OTTHUMP00000025467|chondroitin 4-O-sulfotransferase 2
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Interactome
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Pathway
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