PI4KII 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PI4KII full-length
Host
Human
Theoretical MW (kDa)
54
Interspecies Antigen Sequence
Mouse (95); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PI4KII antibody (H00055361-B01) by Western Blots.
SDS-PAGE Gel
PI4K2A transfected lysate.
Western Blot
Lane 1: PI4K2A transfected lysate ( 54 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PI4K2A
Entrez GeneID
55361GeneBank Accession#
NM_018425Protein Accession#
NP_060895Gene Name
PI4K2A
Gene Alias
DKFZp761G1923, PI4KII, PIK42A, RP11-548K23.6
Gene Description
phosphatidylinositol 4-kinase type 2 alpha
Omim ID
609763Gene Ontology
HyperlinkGene Summary
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM
Other Designations
OTTHUMP00000020224|phosphatidylinositol 4-kinase type II (PI4KII)
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Interactome
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