RFK 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RFK transfected lysate.
Western Blot
Lane 1: RFK transfected lysate ( 17.93 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RFK full-length
Host
Human
Theoretical MW (kDa)
17.93
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RFK antibody (H00055312-B01) by Western Blots.
SDS-PAGE Gel
RFK transfected lysate.
Western Blot
Lane 1: RFK transfected lysate ( 17.93 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RFK
Entrez GeneID
55312GeneBank Accession#
NM_018339.3Protein Accession#
NP_060809.2Gene Name
RFK
Gene Alias
FLJ11149, RIFK
Gene Description
riboflavin kinase
Gene Ontology
HyperlinkGene Summary
Riboflavin kinase (RFK) is an essential enzyme catalyzing the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM
Other Designations
0610038L10Rik|ATP:riboflavin 5'-phosphotransferase|flavokinase
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