RFK (Human) Recombinant Protein (P01)
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Specification
Product Description
Human RFK full-length ORF ( AAH07069, 1 a.a. - 162 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MPRADCIMRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
43.56
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — RFK
Entrez GeneID
55312GeneBank Accession#
BC007069Protein Accession#
AAH07069Gene Name
RFK
Gene Alias
FLJ11149, RIFK
Gene Description
riboflavin kinase
Gene Ontology
HyperlinkGene Summary
Riboflavin kinase (RFK) is an essential enzyme catalyzing the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM
Other Designations
0610038L10Rik|ATP:riboflavin 5'-phosphotransferase|flavokinase
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Interactome
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Pathway
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Publication Reference
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A Bifunctional Molecule as an Artificial Flavin Mononucleotide Cyclase and a Chemosensor for Selective Fluorescent Detection of Flavins.
Rhee HW, Choi SJ, Yoo SH, Jang YO, Park HH, Pinto RM, Cameselle JC, Sandoval FJ, Roje S, Han K, Chung DS, Suh J, Hong JI.
Journal of the American Chemical Society 2009 Jul; 131(29):10107.
Application:Fluorescent real-time assay, Recombinant protein.
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A Bifunctional Molecule as an Artificial Flavin Mononucleotide Cyclase and a Chemosensor for Selective Fluorescent Detection of Flavins.
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