TYW1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TYW1 full-length
Host
Human
Theoretical MW (kDa)
42.35
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TYW1 antibody (H00055253-B01) by Western Blots.
SDS-PAGE Gel
RSAFD1 transfected lysate.
Western Blot
Lane 1: RSAFD1 transfected lysate ( 42.35 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TYW1
Entrez GeneID
55253GeneBank Accession#
BC051888.1Protein Accession#
-Gene Name
TYW1
Gene Alias
FLJ10900, MGC23001, MGC60291, RSAFD1, TYW1A, YPL207W
Gene Description
tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)
Omim ID
611243Gene Ontology
HyperlinkGene Summary
Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM
Other Designations
radical S-adenosyl methionine and flavodoxin domains 1|tRNA-yW synthesizing protein 1 homolog A
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Interactome
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