TYW1 monoclonal antibody (M01A), clone 1G5
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant TYW1.
Immunogen
TYW1 (AAH51888, 1 a.a. ~ 384 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDPSADTWDLFSPLISLWINRFYIYLGFAVSISLWICVQIVIKTQGKNLQEKSVPKAAQDLMTNGYVSLQEKDIFVSGVKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQVHAPRERRLLQTHILWN
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (67.98 KDa) .
Storage Buffer
In ascites fluid
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of TYW1 expression in transfected 293T cell line by RSAFD1 monoclonal antibody (M01A), clone 1G5.
Lane 1: TYW1 transfected lysate (Predicted MW: 43.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — TYW1
Entrez GeneID
55253GeneBank Accession#
BC051888Protein Accession#
AAH51888Gene Name
TYW1
Gene Alias
FLJ10900, MGC23001, MGC60291, RSAFD1, TYW1A, YPL207W
Gene Description
tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)
Omim ID
611243Gene Ontology
HyperlinkGene Summary
Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM
Other Designations
radical S-adenosyl methionine and flavodoxin domains 1|tRNA-yW synthesizing protein 1 homolog A
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Interactome
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Disease
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