SMU1 monoclonal antibody (M01), clone 2B12-2F2
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More Files
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Western Blot (Cell lysate)
SMU1 monoclonal antibody (M01), clone 2B12-2F2 Western Blot analysis of SMU1 expression in Hela S3 NE ( Cat # L013V3 ).
Immunofluorescence
Immunofluorescence of monoclonal antibody to SMU1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (82.17 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant SMU1.
Immunogen
SMU1 (AAH02876, 1 a.a. ~ 513 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSIEIESSDVIRLIMQYLKENSLHRALATLQEETTVSLNTVDSIESFVADINSGHWDTVLQAIQSLKLPDKTLIDLYEQVVLELIELRELGAARSLLRQTDPMIMLKQTQPERYIHLENLLARSYFDPREAYPDGSSKEKRRAAIAQALAGEVSVVPPSRLMALLGQALKWQQHQGLLPPGMTIDLFRGKAAVKDVEEEKFPTQLSRHIKFGQKSHVECARFSPDGQYLVTGSVDGFIEVWNFTTGKIRKDLKYQAQDNFMMMDDAVLCMCFSRDTEMLATGAQDGKIKVWKIQSGQCLRRFERAHSKGVTCLSFSKDSSQILSASFDQTIRIHGLKSGKTLKEFRGHSSFVNEATFTQDGHYIISASSDGTVKIWNMKTTECSNTFKSLGSTAGTDITVNSVILLPKNPEHFVVCNRSNTVVIMNMQGQIVRSFSSGKREGGDFVCCALSPRGEWIYCVGEDFVLYCFSTVTGKLERTLTVHEKDVIGIAHHPHQNLIATYSEDGLLKLWKP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Rat (99)
Isotype
IgG2b kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (82.17 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SMU1 monoclonal antibody (M01), clone 2B12-2F2 Western Blot analysis of SMU1 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SMU1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — SMU1
Entrez GeneID
55234GeneBank Accession#
BC002876Protein Accession#
AAH02876Gene Name
SMU1
Gene Alias
BWD, DKFZp761L0916, FLJ10805, FLJ10870, FLJ11970, MGC117363, RP11-54K16.3, SMU-1, fSAP57
Gene Description
smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000021186|brain-enriched WD-repeat protein|functional spliceosome-associated protein 57|smu-1 suppressor of mec-8 and unc-52 homolog
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Interactome
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Disease
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Publication Reference
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Loss of Smu1 function de-represses DNA replication and over-activates ATR-dependent replication checkpoint.
Surnameren G, Surnameliu G, Surnameguo G, Surnamewang G, Surnamema G, Surnamezeng G, Surnameshao G, Surnameyang G, Surnametang G, Surnamewang G, Surnameliu G, Surnameli G.
Biochemical and Biophysical Research Communications 2013 Jun; 436(2):192.
Application：IF, WB-Ce, WB-Tr, Human, 293T, U2OS cells.
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Loss of Smu1 function de-represses DNA replication and over-activates ATR-dependent replication checkpoint.
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