APPL2 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
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Western Blot (Tissue lysate)
APPL2 MaxPab polyclonal antibody. Western Blot analysis of APPL2 expression in rat brain.
Western Blot (Transfected lysate)
Western Blot analysis of APPL2 expression in transfected 293T cell line (H00055198-T01) by APPL2 MaxPab polyclonal antibody.
Lane 1: APPL2 transfected lysate(73.04 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to APPL2 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human APPL2 protein.
Immunogen
APPL2 (AAH33731.1, 1 a.a. ~ 664 a.a) full-length human protein.
Sequence
MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEKQNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEHDLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINFFKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQKAGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGKSGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNSEMENENDKIVPKVTASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLLQQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFELTSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLMLSIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA
Host
Mouse
Reactivity
Human, Rat
Interspecies Antigen Sequence
Mouse (92); Rat (92)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
APPL2 MaxPab polyclonal antibody. Western Blot analysis of APPL2 expression in rat brain.Western Blot (Transfected lysate)
Western Blot analysis of APPL2 expression in transfected 293T cell line (H00055198-T01) by APPL2 MaxPab polyclonal antibody.
Lane 1: APPL2 transfected lysate(73.04 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to APPL2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — APPL2
Entrez GeneID
55198GeneBank Accession#
BC033731.1Protein Accession#
AAH33731.1Gene Name
APPL2
Gene Alias
DIP13B, FLJ10659
Gene Description
adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
Omim ID
606231Gene Ontology
HyperlinkGene Summary
phosphotyrosine interaction
Other Designations
DIP13 beta|adaptor protein containing PH domain, PTB domain and leucine zipper motif 2
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Interactome
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Publication Reference
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Embryonic stem cells are devoid of macropinocytosis, a trafficking pathway for activin a in differentiated cells.
Nikoleta Kostopoulou, Sofia Bellou, Eleni Bagli, Maria Markou, Eleftherios Kostaras, Marko Hyvönen, Yiannis Kalaidzidis, Angelos Papadopoulos, Varvara Chalmantzi, Athena Kyrkou, Ekaterini Panopoulou, Theodore Fotsis, Carol Murphy.
Journal of Cell Science 2021 Jul; 134(13):jcs246892.
Application：IF, Human, HUVECs.
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Activation of hypothalamic RIP-Cre neurons promotes beiging of WAT via sympathetic nervous system.
Wang B, Li A, Li X, Ho PW, Wu D, Wang X, Liu Z, Wu KK, Yau SS, Xu A, Cheng KK.
EMBO reports 2018 Apr; 19(4):e44977.
Application：IHC, Mouse, Hypothalamic VMH region.
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Cellular, structural and functional cardiac remodelling following pressure overload and unloading.
Dadson K, Kovacevic V, Rengasamy P, Kim GH, Boo S, Li RK, George I, Schulze PC, Hinz B, Sweeney G.
International Journal of Cardiology 2016 Aug; 216:32.
Application：WB-Ti, Mouse, Mouse heart.
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The adaptor protein APPL1 increases glycogen accumulation in rat skeletal muscle through activation of the PI3-kinase signalling pathway.
Cleasby ME, Lau Q, Polkinghorne E, Patel SA, Leslie SJ, Turner N, Cooney GJ, Xu A, Kraegen EW.
J Endocrinol 2011 May; 210:81.
Application：WB-Ti, Rat, Muscle.
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Embryonic stem cells are devoid of macropinocytosis, a trafficking pathway for activin a in differentiated cells.
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