PNPO 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PNPO full-length
Host
Human
Theoretical MW (kDa)
28.82
Interspecies Antigen Sequence
Mouse (90); Rat (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PNPO antibody (H00055163-B01) by Western Blots.
SDS-PAGE Gel
PNPO transfected lysate.
Western Blot
Lane 1: PNPO transfected lysate ( 28.82 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PNPO
Entrez GeneID
55163GeneBank Accession#
NM_018129.1Protein Accession#
NP_060599.1Gene Name
PNPO
Gene Alias
FLJ10535, PDXPO
Gene Description
pyridoxamine 5'-phosphate oxidase
Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. [provided by RefSeq
Other Designations
pyridoxal 5'-phosphate synthase|pyridoxine 5'-phosphate oxidase
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Interactome
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Disease
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