MED9 monoclonal antibody (M03), clone 7F1
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant MED9.
Immunogen
MED9 (NP_060489.1, 1 a.a. ~ 146 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MASAGVAAGRQAEDVLPPTSDQPLPDTKPLPPPQPPPVPAPQPQQSPAPRPQSPARAREEENYSFLPLVHNIIKCMDKDSPEVHQDLNALKSKFQEMRKLISTMPGIHLSPEQQQQQLQSLREQVRTKNELLQKYKSLCMFEIPKE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (78); Rat (78)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (42.8 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MED9 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MED9 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MED9
Entrez GeneID
55090GeneBank Accession#
NM_018019.2Protein Accession#
NP_060489.1Gene Name
MED9
Gene Alias
FLJ10193, MED25, MGC138234
Gene Description
mediator complex subunit 9
Omim ID
609878Gene Ontology
HyperlinkGene Summary
The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq
Other Designations
mediator of RNA polymerase II transcription, subunit 9 homolog|mediator subunit 25
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