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TRMT12 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00055039-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-TRMT12 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 50.2
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-TRMT12 antibody (H00055039-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00055039-T01
    TRMT12 transfected lysate.
    Western Blot
    QC Testing of H00055039-T01
    Lane 1: TRMT12 transfected lysate ( 50.2 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • TRMT12
  • Gene Alias:
  • FLJ20772,TRM12,TYW2
  • Gene Description:
  • tRNA methyltransferase 12 homolog (S. cerevisiae)
  • Gene Summary:
  • Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM
  • Other Designations:
  • homolog of yeast tRNA methyltransferase,homolog of yeast tRNA methyltransferase 12,tRNA methyltranferase 12 homolog,tRNA-yW synthesizing protein 2
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