TRMT12 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TRMT12 transfected lysate.
Western Blot
Lane 1: TRMT12 transfected lysate ( 50.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TRMT12 full-length
Host
Human
Theoretical MW (kDa)
50.2
Interspecies Antigen Sequence
Mouse (81); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TRMT12 antibody (H00055039-B01) by Western Blots.
SDS-PAGE Gel
TRMT12 transfected lysate.
Western Blot
Lane 1: TRMT12 transfected lysate ( 50.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TRMT12
Entrez GeneID
55039GeneBank Accession#
BC011713Protein Accession#
AAH11713.1Gene Name
TRMT12
Gene Alias
FLJ20772, TRM12, TYW2
Gene Description
tRNA methyltransferase 12 homolog (S. cerevisiae)
Omim ID
611244Gene Ontology
HyperlinkGene Summary
Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM
Other Designations
homolog of yeast tRNA methyltransferase|homolog of yeast tRNA methyltransferase 12|tRNA methyltranferase 12 homolog|tRNA-yW synthesizing protein 2
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