TRMT12 mouse monoclonal antibody (hybridoma)
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant TRMT12.
Immunogen
TRMT12 (AAH11713.1, 1 a.a. ~ 448 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MRENVVVSNMERESGKPVAVVAVVTEPRFTQRYREYLQRQKLFDTQHRVEKMPDGSVALPVLGETLPEQHLQELRNRVAPGSPCMLTQLPDPVPSKRAQGCSPAQKLCLEVSRWVEGRGVKWSAELEADLPRSWQRHGNLLLLSEDCFQAKQWKNLGPELWETVALALGVQRLAKRGRVSPDGTRTPAVTLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLSCAGEVLVDLYAGIGYFTLPFLVHAGAAFVHACEWNPHAVVALRNNLEINGVADRCQIHFGDNRKLKLSNIADRVILGLIPSSEEGWPIACQVLRQDAGGILHIHQNVESFPGKNLQALGVSKVEKEHWLYPQQITTNQWKNGATRDSRGKMLSPATKPEWQRWAESAETRIATLLQQVHGKPWKTQILHIQPVKSYAPHVDHIVLDLECCPCPSVG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81); Rat (82)
Quality Control Testing
Antibody reactivity and specificity confirmed by ELISA and Western Blot.
Deliverables
Up to 5 positive hybridoma clones will be delivered to customer in the cryotube format.
Note
Customer should check the viability of the hybridomas within one month from the date of receipt. Fee-for-service of long term hybridoma storage can be performed upon customer's request.
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Applications
Western Blot (Transfected lysate)
Western Blot (Recombinant protein)
ELISA
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Gene Info — TRMT12
Entrez GeneID
55039GeneBank Accession#
BC011713.2Protein Accession#
AAH11713.1Gene Name
TRMT12
Gene Alias
FLJ20772, TRM12, TYW2
Gene Description
tRNA methyltransferase 12 homolog (S. cerevisiae)
Omim ID
611244Gene Ontology
HyperlinkGene Summary
Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM
Other Designations
homolog of yeast tRNA methyltransferase|homolog of yeast tRNA methyltransferase 12|tRNA methyltranferase 12 homolog|tRNA-yW synthesizing protein 2
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Interactome
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