RNF125 monoclonal antibody (M03), clone 1D3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RNF125.
Immunogen
RNF125 (NP_060301, 143 a.a. ~ 231 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
FCQRELYEDSLLDHCITHHRSERRPVFCPLCRLIPDENPSSFSGSLIRHLQVSHTLFYDDFIDFNIIEEALIRRVLDRSLLEYVNHSNT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Rat (86)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RNF125 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to RNF125 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — RNF125
Entrez GeneID
54941GeneBank Accession#
NM_017831Protein Accession#
NP_060301Gene Name
RNF125
Gene Alias
FLJ20456, MGC21737, TRAC1
Gene Description
ring finger protein 125
Omim ID
610432Gene Ontology
HyperlinkGene Summary
This gene encodes a novel E3 ubiquitin ligase that contains an N-terminal RING finger domain. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway. [provided by RefSeq
Other Designations
T-cell ring protein identified in activation screen
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