APTX 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
APTX transfected lysate.
Western Blot
Lane 1: APTX transfected lysate ( 29.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-APTX full-length
Host
Human
Theoretical MW (kDa)
29.1
Interspecies Antigen Sequence
Mouse (83); Rat (79)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-APTX antibody (H00054840-B01) by Western Blots.
SDS-PAGE Gel
APTX transfected lysate.
Western Blot
Lane 1: APTX transfected lysate ( 29.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — APTX
Entrez GeneID
54840GeneBank Accession#
NM_175072.1Protein Accession#
NP_778242.1Gene Name
APTX
Gene Alias
AOA, AOA1, AXA1, EAOH, EOAHA, FHA-HIT, FLJ20157, MGC1072
Gene Description
aprataxin
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000021188|ataxia 1, early onset with hypoalbuminemia
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Interactome
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Disease
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