APTX monoclonal antibody (M02), clone 2H6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of APTX expression in transfected 293T cell line by APTX monoclonal antibody (M02), clone 2H6.
Lane 1: APTX transfected lysate (Predicted MW: 29.1 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged APTX is 3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.52 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant APTX.
Immunogen
APTX (NP_778241.1, 69 a.a. ~ 166 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KLRFRLGYHAIPSMSHVHLHVISQDFDSPCLKNKKHWNSFNTEYFLESQAVIEMVQEAGRVTVRDGMPELLKLPLRCHECQQLLPSIPQLKEHLRKHW
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (83); Rat (79)
Isotype
IgG2a Lambda
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of APTX expression in transfected 293T cell line by APTX monoclonal antibody (M02), clone 2H6.
Lane 1: APTX transfected lysate (Predicted MW: 29.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged APTX is 3 ng/ml as a capture antibody.ELISA
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Gene Info — APTX
Entrez GeneID
54840GeneBank Accession#
NM_175071Protein Accession#
NP_778241.1Gene Name
APTX
Gene Alias
AOA, AOA1, AXA1, EAOH, EOAHA, FHA-HIT, FLJ20157, MGC1072
Gene Description
aprataxin
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000021188|ataxia 1, early onset with hypoalbuminemia
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Interactome
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Disease
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