MED18 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MED18 transfected lysate.
Western Blot
Lane 1: MED18 transfected lysate ( 23.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MED18 full-length
Host
Human
Theoretical MW (kDa)
23.7
Interspecies Antigen Sequence
Mouse (98); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MED18 antibody (H00054797-D01P) by Western Blots.
SDS-PAGE Gel
MED18 transfected lysate.
Western Blot
Lane 1: MED18 transfected lysate ( 23.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MED18
Entrez GeneID
54797GeneBank Accession#
BC002694Protein Accession#
AAH02694.1Gene Name
MED18
Gene Alias
FLJ20045, p28b
Gene Description
mediator complex subunit 18
Gene Ontology
HyperlinkGene Summary
MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM
Other Designations
OTTHUMP00000003645|TRAP/mediator complex subunit p28b|mediator of RNA polymerase II transcription, subunit 18 homolog
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