PCSK4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PCSK4 transfected lysate.
Western Blot
Lane 1: PCSK4 transfected lysate ( 26.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PCSK4 full-length
Host
Human
Theoretical MW (kDa)
26.1
Interspecies Antigen Sequence
Mouse (65); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PCSK4 antibody (H00054760-B02) by Western Blots.
SDS-PAGE Gel
PCSK4 transfected lysate.
Western Blot
Lane 1: PCSK4 transfected lysate ( 26.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PCSK4
Entrez GeneID
54760GeneBank Accession#
BC036354Protein Accession#
AAH36354Gene Name
PCSK4
Gene Alias
DKFZp434B217, MGC34749, PC4, SPC5
Gene Description
proprotein convertase subtilisin/kexin type 4
Omim ID
600487Gene Ontology
HyperlinkGene Summary
Proprotein convertases, including PCSK4, are calcium-dependent serine proteases related to bacterial subtilisins and to yeast kexin. These enzymes process precursor proteins to their active forms by selective cleavage of the polypeptide at sites following paired basic amino acids. In mammals, this family comprises PC1 (MIM 162150), PC2 (MIM 162151), PC4, PC5 (MIM 600488), furin (FUR; MIM 136950), and PACE4 (MIM 167405). Substrates for these enzymes range from prohormones to precursors for growth factors to cell surface receptors and viral surface glycoproteins (Cao et al., 2001 [PubMed 11776387]).[supplied by OMIM
Other Designations
OTTHUMP00000158677
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