P4HTM 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
P4HTM transfected lysate.
Western Blot
Lane 1: P4HTM transfected lysate ( 62.04 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-P4HTM full-length
Host
Human
Theoretical MW (kDa)
62.04
Interspecies Antigen Sequence
Mouse (87); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-P4HTM antibody (H00054681-B01) by Western Blots.
SDS-PAGE Gel
P4HTM transfected lysate.
Western Blot
Lane 1: P4HTM transfected lysate ( 62.04 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — P4HTM
Entrez GeneID
54681GeneBank Accession#
NM_177938.2Protein Accession#
NP_808807.2Gene Name
P4HTM
Gene Alias
EGLN4, HIFPH4, P4H-TM, PH-4, PH4, PHD4
Gene Description
prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000082685|P4H with transmembrane domain|Prolyl hydroxlase domain-containing 4|hypoxia inducible factor prolyl 4 hydroxylase|hypoxia-inducible factor prolyl 4-hydroxylase|proline 4-hydroxylase
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