CROT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CROT transfected lysate.
Western Blot
Lane 1: CROT transfected lysate ( 67.43 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CROT full-length
Host
Human
Theoretical MW (kDa)
67.43
Interspecies Antigen Sequence
Mouse (85); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CROT antibody (H00054677-B01) by Western Blots.
SDS-PAGE Gel
CROT transfected lysate.
Western Blot
Lane 1: CROT transfected lysate ( 67.43 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CROT
Entrez GeneID
54677GeneBank Accession#
NM_021151.2Protein Accession#
NP_066974.2Gene Name
CROT
Gene Alias
COT
Gene Description
carnitine O-octanoyltransferase
Omim ID
606090Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described
Other Designations
peroxisomal carnitine O-octanoyltransferase|peroxisomal carnitine acyltransferase|peroxisomal carnitine octanoyltransferase
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Interactome
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Disease
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