Western blot analysis of SCAND2 over-expressed 293 cell line, cotransfected with SCAND2 Validated Chimera RNAi ( Cat # H00054581-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SCAND2 monoclonal antibody (M01), clone 7B12 (Cat # H00054581-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
The SCAN domain is a highly conserved, leucine-rich motif of approximately 60 aa originally found within a subfamily of zinc finger proteins. This gene belongs to a family of genes that encode an isolated SCAN domain, but no zinc finger motif. Functional studies have established that the SCAN box is a protein interaction domain that mediates both hetero- and homoprotein associations, and maybe involved in regulation of transcriptional activity. Multiple transcript variants which encode the same isoform but differ only in their 3' UTRs, and another variant which encodes a distinct isoform have been described for this gene.