DDX4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DDX4 full-length
Host
Human
Theoretical MW (kDa)
76.01
Interspecies Antigen Sequence
Mouse (84); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDX4 antibody (H00054514-B01) by Western Blots.
SDS-PAGE Gel
DDX4 transfected lysate.
Western Blot
Lane 1: DDX4 transfected lysate ( 76.01 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DDX4
Entrez GeneID
54514GeneBank Accession#
BC047455.1Protein Accession#
AAH47455.1Gene Name
DDX4
Gene Alias
MGC111074, VASA
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Omim ID
605281Gene Ontology
HyperlinkGene Summary
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq
Other Designations
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4|OTTHUMP00000122546
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