TOLLIP monoclonal antibody (M01), clone 1A5-2A3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant TOLLIP.
Immunogen
TOLLIP (AAH04420.1, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (55.88 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TOLLIP monoclonal antibody (M01), clone 1A5-2A3 Western Blot analysis of TOLLIP expression in 293 ( Cat # L026V1 ).Western Blot (Transfected lysate)
Western Blot analysis of TOLLIP expression in transfected 293T cell line by TOLLIP monoclonal antibody (M01), clone 1A5-2A3.
Lane 1: TOLLIP transfected lysate (Predicted MW: 30.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of TOLLIP transfected lysate using anti-TOLLIP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TOLLIP monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TOLLIP is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — TOLLIP
Entrez GeneID
54472GeneBank Accession#
BC004420Protein Accession#
AAH04420.1Gene Name
TOLLIP
Gene Alias
FLJ33531, IL-1RAcPIP
Gene Description
toll interacting protein
Omim ID
606277Gene Ontology
HyperlinkOther Designations
Toll-interacting protein|adapter protein
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Interactome
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Pathway
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Disease
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