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Last updated: 2017/4/23
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TOLLIP monoclonal antibody (M01), clone 1A5-2A3

  • Catalog # : H00054472-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full length recombinant TOLLIP.
  • Immunogen:
  • TOLLIP (AAH04420.1, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00054472-M01
    Western Blot detection against Immunogen (55.88 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • TOLLIP monoclonal antibody (M01), clone 1A5-2A3 Western Blot analysis of TOLLIP expression in 293 ( Cat # L026V1 ).
  • PDF DownloadProtocol Download
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of TOLLIP expression in transfected 293T cell line by TOLLIP monoclonal antibody (M01), clone 1A5-2A3.

    Lane 1: TOLLIP transfected lysate (Predicted MW: 30.3 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation of TOLLIP transfected lysate using anti-TOLLIP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TOLLIP monoclonal antibody.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged TOLLIP is approximately 1ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • TOLLIP
  • Gene Alias:
  • FLJ33531,IL-1RAcPIP
  • Gene Description:
  • toll interacting protein
  • Other Designations:
  • Toll-interacting protein,adapter protein
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