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FBXW5 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00054461-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-FBXW5 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 17.6
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-FBXW5 antibody (H00054461-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00054461-T01
    FBXW5 transfected lysate.
    Western Blot
    QC Testing of H00054461-T01
    Lane 1: FBXW5 transfected lysate ( 17.6 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • FBXW5
  • Gene Alias:
  • DKFZp434B205,Fbw5,MGC20962,RP11-229P13.10
  • Gene Description:
  • F-box and WD repeat domain containing 5
  • Gene Summary:
  • This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains WD-40 domains, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene, however, they were found to be nonsense-mediated mRNA decay (NMD) candidates, hence not represented. [provided by RefSeq
  • Other Designations:
  • F-box and WD-40 domain protein 5,OTTHUMP00000022659,WD repeat-containing F-box protein FBW5
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