HAO1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
HAO1 transfected lysate.
Western Blot
Lane 1: HAO1 transfected lysate ( 40.81 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HAO1 full-length
Host
Human
Theoretical MW (kDa)
40.81
Interspecies Antigen Sequence
Mouse (89); Rat (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HAO1 antibody (H00054363-B01) by Western Blots.
SDS-PAGE Gel
HAO1 transfected lysate.
Western Blot
Lane 1: HAO1 transfected lysate ( 40.81 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HAO1
Entrez GeneID
54363GeneBank Accession#
NM_017545.2Protein Accession#
NP_060015.1Gene Name
HAO1
Gene Alias
GOX, GOX1, HAOX1, MGC142225, MGC142227
Gene Description
hydroxyacid oxidase (glycolate oxidase) 1
Omim ID
605023Gene Ontology
HyperlinkGene Summary
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq
Other Designations
(S)-2-hydroxy-acid oxidase|OTTHUMP00000030231|glycolate oxidase|hydroxyacid oxidase 1
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