WNT4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
WNT4 transfected lysate.
Western Blot
Lane 1: WNT4 transfected lysate ( 38.72 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WNT4 full-length
Host
Human
Theoretical MW (kDa)
38.72
Interspecies Antigen Sequence
Mouse (98); Rat (98)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WNT4 antibody (H00054361-B02) by Western Blots.
SDS-PAGE Gel
WNT4 transfected lysate.
Western Blot
Lane 1: WNT4 transfected lysate ( 38.72 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WNT4
Entrez GeneID
54361GeneBank Accession#
NM_030761Protein Accession#
NP_110388Gene Name
WNT4
Gene Alias
SERKAL, WNT-4
Gene Description
wingless-type MMTV integration site family, member 4
Gene Ontology
HyperlinkGene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq
Other Designations
OTTHUMP00000002937|OTTHUMP00000044725|WNT-4 protein
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Interactome
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