POLE3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
POLE3 transfected lysate
Western Blot
Lane 1: POLE3 transfected lysate ( 16.9 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-POLE3 full-length
Host
Human
Theoretical MW (kDa)
16.9
Interspecies Antigen Sequence
Mouse (93); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-POLE3 antibody (H00054107-B02) by Western Blots.
SDS-PAGE Gel
POLE3 transfected lysate
Western Blot
Lane 1: POLE3 transfected lysate ( 16.9 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — POLE3
Entrez GeneID
54107GeneBank Accession#
BC003166Protein Accession#
AAH03166Gene Name
POLE3
Gene Alias
CHARAC17, CHRAC17, YBL1, p17
Gene Description
polymerase (DNA directed), epsilon 3 (p17 subunit)
Omim ID
607267Gene Ontology
HyperlinkGene Summary
POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM
Other Designations
DNA polymerase epsilon p17 subunit|DNA polymerase epsilon subunit 3|OTTHUMP00000021954|OTTHUMP00000021955|arsenic transactivated protein|chromatin accessibility complex 17|histone fold protein CHRAC17
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