PELO monoclonal antibody (M03), clone 2C2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PELO expression in transfected 293T cell line by PELO monoclonal antibody (M03), clone 2C2.
Lane 1: PELO transfected lysate (Predicted MW: 43.4 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PELO transfected lysate using anti-PELO monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PELO monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PELO is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.19 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PELO.
Immunogen
PELO (NP_057030.3, 291 a.a. ~ 385 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RAFYGLKQVEKANEAMAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLHVSGEQLSQLTGVAAILRFPVPELSDQEGDSSSEED
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.19 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PELO expression in transfected 293T cell line by PELO monoclonal antibody (M03), clone 2C2.
Lane 1: PELO transfected lysate (Predicted MW: 43.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PELO transfected lysate using anti-PELO monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PELO monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PELO is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — PELO
Entrez GeneID
53918GeneBank Accession#
NM_015946Protein Accession#
NP_057030.3Gene Name
PELO
Gene Alias
CGI-17, PRO1770
Gene Description
pelota homolog (Drosophila)
Omim ID
605757Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division. [provided by RefSeq
Other Designations
OTTHUMP00000122446|pelota homolog
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Interactome
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Disease
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