MYO3A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
MYO3A transfected lysate.
Western Blot
Lane 1: MYO3A transfected lysate ( 27.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MYO3A full-length
Host
Human
Theoretical MW (kDa)
27.28
Interspecies Antigen Sequence
Mouse (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MYO3A antibody (H00053904-B01) by Western Blots.
SDS-PAGE Gel
MYO3A transfected lysate.
Western Blot
Lane 1: MYO3A transfected lysate ( 27.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MYO3A
Entrez GeneID
53904GeneBank Accession#
BC045538.1Protein Accession#
-Gene Name
MYO3A
Gene Alias
DFNB30
Gene Description
myosin IIIA
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the myosin superfamily. Myosins are actin-dependent motor proteins and are categorized into conventional myosins (class II) and unconventional myosins (classes I and III through XV) based on their variable C-terminal cargo-binding domains. Class III myosins, such as this one, have a kinase domain N-terminal to the conserved N-terminal motor domains and are expressed in photoreceptors. The protein encoded by this gene plays an important role in hearing in humans. Three different recessive, loss of function mutations in the encoded protein have been shown to cause nonsyndromic progressive hearing loss. Expression of this gene is highly restricted, with the strongest expression in retina and cochlea. [provided by RefSeq
Other Designations
OTTHUMP00000019339
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Interactome
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Disease
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