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MBD3 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00053615-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MBD3 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 32.12
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MBD3 antibody (H00053615-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00053615-T01
    MBD3 transfected lysate.
    Western Blot
    QC Testing of H00053615-T01
    Lane 1: MBD3 transfected lysate ( 32.12 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • MBD3
  • Gene Alias:
  • -
  • Gene Description:
  • methyl-CpG binding domain protein 3
  • Gene Summary:
  • DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq
  • Other Designations:
  • -
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