MBD3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MBD3 transfected lysate.
Western Blot
Lane 1: MBD3 transfected lysate ( 32.12 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MBD3 full-length
Host
Human
Theoretical MW (kDa)
32.12
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MBD3 antibody (H00053615-B01) by Western Blots.
SDS-PAGE Gel
MBD3 transfected lysate.
Western Blot
Lane 1: MBD3 transfected lysate ( 32.12 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MBD3
Entrez GeneID
53615GeneBank Accession#
NM_003926.5Protein Accession#
NP_003917.1Gene Name
MBD3
Gene Alias
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Gene Description
methyl-CpG binding domain protein 3
Omim ID
603573Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq
Other Designations
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