SPA17 293T Cell Transient Overexpression Lysate(Denatured)

Catalog # H00053340-T01

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Size:100 uL
Price: USD $ 247.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
SDS-PAGE Gel
QC Test

SDS-PAGE Gel

SPA17 transfected lysate.

Western Blot
QC Test

Western Blot

Lane 1: SPA17 transfected lysate ( 16.72 KDa)
Lane 2: Non-transfected lysate.

  • Specification

    Transfected Cell Line

    293T

    Plasmid

    pCMV-SPA17 full-length

    Host

    Human

    Theoretical MW (kDa)

    16.72

    Interspecies Antigen Sequence

    Mouse (72); Rat (72)

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-SPA17 antibody (H00053340-B01) by Western Blots.

    SDS-PAGE Gel

    SPA17 transfected lysate.

    Western Blot

    Lane 1: SPA17 transfected lysate ( 16.72 KDa)
    Lane 2: Non-transfected lysate.

    Storage Buffer

    1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

  • Gene Info — SPA17

    Entrez GeneID

    53340

    GeneBank Accession#

    NM_017425.2

    Protein Accession#

    -

    Gene Name

    SPA17

    Gene Alias

    SP17, SP17-1

    Gene Description

    sperm autoantigenic protein 17

    Omim ID

    608621

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22

    Other Designations

    sperm protein 17

  • Interactome
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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