CALML5 monoclonal antibody (M16), clone 2F10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of CALML5 expression in transfected 293T cell line by CALML5 monoclonal antibody (M16), clone 2F10.
Lane 1: CALML5 transfected lysate(15.9 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CALML5 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CALML5 is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (41.8 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant CALML5.
Immunogen
CALML5 (AAH39172, 1 a.a. ~ 146 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAGELTPEEEAQYKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDGDGDGEISFQEFLTAARKARAGLEDLQVAFRAFDQDGDGHITVDELRRAMAGLGQPLPQEELDAMIREADVDQDGRVNYEEFARMLAQE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (51); Rat (51)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (41.8 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CALML5 expression in transfected 293T cell line by CALML5 monoclonal antibody (M16), clone 2F10.
Lane 1: CALML5 transfected lysate(15.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to CALML5 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CALML5 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — CALML5
Entrez GeneID
51806GeneBank Accession#
BC039172Protein Accession#
AAH39172Gene Name
CALML5
Gene Alias
CLSP
Gene Description
calmodulin-like 5
Omim ID
605183Gene Ontology
HyperlinkGene Summary
This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes. [provided by RefSeq
Other Designations
OTTHUMP00000019005|calmodulin-like skin protein
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Interactome
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Pathway
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