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COQ3 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00051805-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-COQ3 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 40.7
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-COQ3 antibody (H00051805-B01) by Western Blots.
    Western Blot
    QC Testing of H00051805-T01
    Lane 1: COQ3 transfected lysate ( 40.7 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00051805-T01
    COQ3 transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Protein Accession#:
  • -
  • Gene Name:
  • COQ3
  • Gene Alias:
  • UG0215E05,bA9819.1
  • Gene Description:
  • coenzyme Q3 homolog, methyltransferase (S. cerevisiae)
  • Gene Summary:
  • Ubiquinone, also known as coenzyme Q, or Q, is a critical component of the electron transport pathways of both eukaryotes and prokaryotes (Jonassen and Clarke, 2000 [PubMed 10777520]). This lipid consists of a hydrophobic isoprenoid tail and a quinone head group. The tail varies in length depending on the organism, but its purpose is to anchor coenzyme Q to the membrane. The quinone head group is responsible for the activity of coenzyme Q in the respiratory chain. The S. cerevisiae COQ3 gene encodes an O-methyltransferase required for 2 steps in the biosynthetic pathway of coenzyme Q. This enzyme methylates an early coenzyme Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-ubiquinone to coenzyme Q. The COQ3 gene product is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol.[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000016892,methyltransferase COQ3
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