COQ3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
COQ3 transfected lysate.
Western Blot
Lane 1: COQ3 transfected lysate ( 40.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-COQ3 full-length
Host
Human
Theoretical MW (kDa)
40.7
Interspecies Antigen Sequence
Mouse (73); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-COQ3 antibody (H00051805-B01) by Western Blots.
SDS-PAGE Gel
COQ3 transfected lysate.
Western Blot
Lane 1: COQ3 transfected lysate ( 40.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — COQ3
Entrez GeneID
51805GeneBank Accession#
BC063463.1Protein Accession#
-Gene Name
COQ3
Gene Alias
UG0215E05, bA9819.1
Gene Description
coenzyme Q3 homolog, methyltransferase (S. cerevisiae)
Omim ID
605196Gene Ontology
HyperlinkGene Summary
Ubiquinone, also known as coenzyme Q, or Q, is a critical component of the electron transport pathways of both eukaryotes and prokaryotes (Jonassen and Clarke, 2000 [PubMed 10777520]). This lipid consists of a hydrophobic isoprenoid tail and a quinone head group. The tail varies in length depending on the organism, but its purpose is to anchor coenzyme Q to the membrane. The quinone head group is responsible for the activity of coenzyme Q in the respiratory chain. The S. cerevisiae COQ3 gene encodes an O-methyltransferase required for 2 steps in the biosynthetic pathway of coenzyme Q. This enzyme methylates an early coenzyme Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-ubiquinone to coenzyme Q. The COQ3 gene product is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol.[supplied by OMIM
Other Designations
OTTHUMP00000016892|methyltransferase COQ3
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Interactome
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